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August 25, 2016

Transparent Mouse

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Abstract of the paper published August 22, 2016 in the journal Nature Methods below.

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Shrinkage-mediated imaging of entire organs and organisms using uDISCO

Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body.

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We developed "ultimate DISCO" (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65%.

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We used uDISCO to image neuronal connections and vasculature from head to toe over 7 cm and to perform unbiased screening of transplanted stem cells within the entire body of adult mice. uDISCO is compatible with diverse labeling methods and archival human tissue, and it can readily be used in various biomedical applications to study organization of large organ systems throughout entire organisms.

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As a reward for reading this far, you get to watch a cool video 

showing a trip into a mouse brain.

[via Business Insider]

August 25, 2016 at 12:01 PM | Permalink | Comments (0)

The Spectacular Nixie Clocks of Dalibor Farny

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Steve Wozniak might be considered the godfather of the Nixie renaissance.

Dalibor Farny (below)

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of the Czech Republic is the majordomo of this movement.

Can he build one for you?

He's standing by to take your call: +420 724 321 571

email: dalibor@farny.cz

August 25, 2016 at 08:01 AM | Permalink | Comments (0)

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